6GY2
Crystal structure of human Plk1-PBD in complex with WSSSLATPPTLSSpTVLI phosphopeptide from BRCA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9840 |
Spacegroup name | P 1 |
Unit cell lengths | 50.000, 56.040, 61.030 |
Unit cell angles | 80.79, 79.23, 65.05 |
Refinement procedure
Resolution | 22.570 - 3.110 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o9w |
RMSD bond length | 0.007 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.570 | 3.160 |
High resolution limit [Å] | 3.106 | 3.106 |
Rmerge | 0.056 | 0.346 |
Rmeas | 0.076 | 0.466 |
Rpim | 0.051 | 0.310 |
Number of reflections | 9934 | 523 |
<I/σ(I)> | 10.1 | 2.3 |
Completeness [%] | 93.7 | 96.9 |
Redundancy | 1.94 | 1.98 |
CC(1/2) | 0.997 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, BisTris, DTT |