6GVW
Crystal structure of the BRCA1-A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.096, 122.645, 431.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.990 - 3.750 |
| R-factor | 0.22896 |
| Rwork | 0.227 |
| R-free | 0.25910 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.147 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.814 |
| High resolution limit [Å] | 3.750 | 3.750 |
| Rmerge | 0.173 | 15.709 |
| Rmeas | 0.175 | 15.860 |
| Number of reflections | 53796 | 2661 |
| <I/σ(I)> | 11.6 | 0.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 51.9 | 52.8 |
| CC(1/2) | 0.952 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 100 mM MES-KOH pH 5.6, 200 mM MgCl2, 8% (w/v) PEG6000 |
