6GVW
Crystal structure of the BRCA1-A complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.096, 122.645, 431.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.990 - 3.750 |
R-factor | 0.22896 |
Rwork | 0.227 |
R-free | 0.25910 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.147 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.814 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.173 | 15.709 |
Rmeas | 0.175 | 15.860 |
Number of reflections | 53796 | 2661 |
<I/σ(I)> | 11.6 | 0.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 51.9 | 52.8 |
CC(1/2) | 0.952 | 0.476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 100 mM MES-KOH pH 5.6, 200 mM MgCl2, 8% (w/v) PEG6000 |