6GMX

pVHL:EloB:EloC in complex with 6-chlorothiochroman-4-one

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 41 2 2
Unit cell lengths	94.000, 94.000, 365.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.000 - 2.533
Rwork	0.188
R-free	0.36090
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BUSTER-TNT
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.000	2.520
High resolution limit [Å]	2.370	2.370
Rmeas	0.240
Number of reflections	67228
<I/σ(I)>	14.7	2.2
Completeness [%]	99.5
Redundancy	12.7
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M Na cacodylate pH 6.0, 0.2 M Mg acetate, 15% PEG3350, 5mM DTT