6GK4
Human NBD1 of CFTR in complex with nanobodies D12 and T8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.940, 55.190, 114.990 |
| Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
| Resolution | 45.160 - 2.910 |
| R-factor | 0.2268 |
| Rwork | 0.246 |
| R-free | 0.29460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 3.014 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.135 | 0.592 |
| Rmeas | 0.161 | 0.703 |
| Number of reflections | 21888 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.985 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000 |
