6GHD

Structural analysis of the ternary complex between lamin A/C, BAF and emerin identifies an interface disrupted in autosomal recessive progeroid diseases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9725
Spacegroup name	P 1
Unit cell lengths	57.770, 62.750, 64.340
Unit cell angles	66.44, 66.55, 88.05

Refinement procedure

Resolution	49.152 - 2.100
R-factor	0.1944
Rwork	0.192
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bzf 1ifr 2odc
RMSD bond length	0.008
RMSD bond angle	0.929
Data reduction software	XDS
Data scaling software	APEX
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.152	6.520
High resolution limit [Å]	2.100	2.100
Rmerge	0.117	0.795
Rmeas	0.139	0.958
Rpim	0.074	0.526
Number of reflections	83934
<I/σ(I)>	5.9
Completeness [%]	95.7
Redundancy	3.52
CC(1/2)	0.992	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	25% PEG3.350, 20mM Tris-BispH5.5, 30mM NaCl, 0.2M NH4SO4