6GE6
X-ray structure of TEAD4(E263A+Y429F mutant) complexed with YAP(wildtype): The role of residual flexibility and water molecules in the adaptation of a bound intrinsically disordered protein to mutations at a binding interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99993 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.902, 58.902, 158.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.490 - 1.800 |
R-factor | 0.2196 |
Rwork | 0.219 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ge3 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.931 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.490 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.085 | 0.812 |
Rmeas | 0.088 | 0.845 |
Total number of observations | 342171 | |
Number of reflections | 26774 | |
<I/σ(I)> | 23 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 12.8 | 13.1 |
CC(1/2) | 0.999 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2M ammonium sulfate, 0.1M Bis-TRIS |