6FYY

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C2)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	FEI FALCON III (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.02
Number of Particle	394672
Software	RELION,REFMAC,EMAN,EPU,Gctf,UCSF Chimera,Coot
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION