6FWH
Acanthamoeba IGPD in complex with R-C348 to 1.7A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.690, 156.100, 117.120 |
Unit cell angles | 90.00, 125.42, 90.00 |
Refinement procedure
Resolution | 95.440 - 1.790 |
R-factor | 0.15129 |
Rwork | 0.149 |
R-free | 0.19016 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ekw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.512 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.440 | 1.840 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.076 | 0.535 |
Rpim | 0.058 | 0.309 |
Number of reflections | 228093 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M calcium acetate and 20 % (w/v) PEG3350 |