6FQQ
Crystal structure of TALE homeobox domain transcription factor TGIF1 double alanine mutant bound to its consensus DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07227 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.058, 93.016, 100.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.110 - 3.250 |
R-factor | 0.217663827964 |
Rwork | 0.215 |
R-free | 0.27291 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fqp |
RMSD bond length | 0.012 |
RMSD bond angle | 1.298 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.110 | 3.360 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.081 | 0.288 |
Number of reflections | 9236 | |
<I/σ(I)> | 4.4 | |
Completeness [%] | 99.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M L-Proline, 0.1M HEPES pH 7.5, 24% v/v PEG 1,000 |