6FP6

Complex of human Cu,Zn SOD1 with the human copper chaperone for SOD1 in a compact conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 32
Unit cell lengths	181.054, 181.054, 141.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.010 - 3.000
R-factor	0.192
Rwork	0.189
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fol 2rsq
RMSD bond length	0.007
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	3.100
High resolution limit [Å]	2.940	2.940
Rmerge	0.165	0.944
Number of reflections	110021
<I/σ(I)>	8.5	1.9
Completeness [%]	100.0	100
Redundancy	5.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M PCTP buffer pH 9.0, 20% (w/v) PEG 3350.