6FMI

pVHL:EloB:EloC in complex with N-((S)-1-((2S,4R)-4-Hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamothioyl) pyrrolidin-1-yl)-1-oxopropan-2-yl)acetamide (ligand 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	C 2 2 21
Unit cell lengths	63.972, 69.863, 356.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.180 - 2.800
R-factor	0.2012
Rwork	0.198
R-free	0.25641
RMSD bond length	0.007
RMSD bond angle	1.172
Data reduction software	XDS
Data scaling software	Aimless (0.5.9)
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	20290
<I/σ(I)>	10
Completeness [%]	99.9
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, MgOAc, Sodium cacodylate, DTT