6FM4
The crystal structure of S. aureus Gyrase complex with ID-130 and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 |
Unit cell lengths | 92.951, 92.951, 407.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.050 - 2.700 |
R-factor | 0.2284 |
Rwork | 0.225 |
R-free | 0.29990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xcs |
RMSD bond length | 0.013 |
RMSD bond angle | 1.674 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.050 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.084 | |
Number of reflections | 51255 | |
<I/σ(I)> | 30.68 | |
Completeness [%] | 99.7 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295.15 | 18% PEG 5000 MME, 0.1 M BISTRIS PH 6.5 |