6FI8

Crystal structure of the IS608 transposase in complex with left end 29-mer DNA hairpin and a 6-mer DNA representing the intact target site: pre-cleavage target capture complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	138.640, 138.640, 117.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.837 - 2.598
R-factor	0.199
Rwork	0.197
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vjv
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.840	2.691
High resolution limit [Å]	2.598	2.598
Rmeas	0.309
Rpim	0.097	0.429
Number of reflections	35947	3525
<I/σ(I)>	6.03	1.4
Completeness [%]	99.7	99.58
Redundancy	9.8	10
CC(1/2)	0.994	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14.6% PEG 3350, 0.19 M calcium acetate