6FHH

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter bound to a 22-mer modified Pol II CTD peptide with serine 5 thiophosphorylated.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97242
Spacegroup name	C 1 2 1
Unit cell lengths	267.770, 147.810, 88.410
Unit cell angles	90.00, 97.08, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.21514
Rwork	0.213
R-free	0.25763
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m3h
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	92066
<I/σ(I)>	14.34	1.87
Completeness [%]	98.6	96.7
Redundancy	2.92	2.69
CC(1/2)	0.996	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Bat FluA polymerase bound to the ends of the RNA promotor (5 prime end nts 1-16, 3 prime end nts 1-18) was co-crystallized in a 1:8 ratio with the thiophospho-serine-5 22-mer Pol II CTD peptide mimic in 0.8 M sodium-potassium phosphate at pH 5.0. Crystals were set at 4 C using seeding.