6FHH

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter bound to a 22-mer modified Pol II CTD peptide with serine 5 thiophosphorylated.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	738	85490.9	1	UniProt (H6QM92) Pfam (PF00603) In PDB	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
2	B	RNA-directed RNA polymerase catalytic subunit	polymer	776	87936.3	1	UniProt (H6QM91) Pfam (PF00602) In PDB	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
3	C	Polymerase basic protein 2	polymer	809	91027.1	1	UniProt (H6QM90) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952) In PDB	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
4	R	RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*U)-3')	polymer	18	5584.3	1		Influenza A virus
5	V	RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*GP*GP*G)-3')	polymer	16	5248.2	1		Influenza A virus
6	X, Y	TYR-SER-PRO-THR-TPS-PRO-SER	polymer	22	2628.5	2		Homo sapiens
7	A, B, C	PHOSPHATE ION	non-polymer		95.0	15	Chemie (PO4)
8	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
9		water	water		18.0	137	Chemie (HOH)

Sequence modifications

A: 1 - 713 (UniProt: H6QM92)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 714	-	expression tag
Ser 715	-	expression tag
Gly 716	-	expression tag
Ser 717	-	expression tag
Gly 718	-	expression tag
Glu 719	-	expression tag
Asn 720	-	expression tag
Leu 721	-	expression tag
Tyr 722	-	expression tag
Phe 723	-	expression tag
Gln 724	-	expression tag

B: 1 - 756 (UniProt: H6QM91)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 757	-	expression tag
Ser 758	-	expression tag
Gly 759	-	expression tag
Ser 760	-	expression tag
Gly 761	-	expression tag
Glu 762	-	expression tag
Asn 763	-	expression tag
Leu 764	-	expression tag
Tyr 765	-	expression tag
Phe 766	-	expression tag
Gln 767	-	expression tag

C: 1 - 760 (UniProt: H6QM90)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 761	-	expression tag
Trp 762	-	expression tag
Ser 763	-	expression tag
His 764	-	expression tag
Pro 765	-	expression tag
Gln 766	-	expression tag
Phe 767	-	expression tag
Glu 768	-	expression tag
Lys 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Gly 772	-	expression tag
Ser 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Gly 776	-	expression tag
Ser 777	-	expression tag
Gly 778	-	expression tag
Gly 779	-	expression tag
Ser 780	-	expression tag
Ala 781	-	expression tag
Trp 782	-	expression tag
Ser 783	-	expression tag
His 784	-	expression tag
Pro 785	-	expression tag
Gln 786	-	expression tag
Phe 787	-	expression tag
Glu 788	-	expression tag
Lys 789	-	expression tag
Gly 790	-	expression tag
Arg 791	-	expression tag
Ser 792	-	expression tag
Gly 793	-	expression tag
Gly 794	-	expression tag
Glu 795	-	expression tag
Asn 796	-	expression tag
Leu 797	-	expression tag
Tyr 798	-	expression tag
Phe 799	-	expression tag
Gln 800	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	280544.0
Non-Polymers*	Number of molecules	16
	Total formula weight	1490.0
All*	Total formula weight	282034.0

*Water molecules are not included.