6FFY
Structure of the mouse SorCS2-NGF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.755, 117.706, 90.000 |
| Unit cell angles | 90.00, 111.87, 90.00 |
Refinement procedure
| Resolution | 60.841 - 3.900 |
| R-factor | 0.2607 |
| Rwork | 0.258 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eax 4msl 1wgo 4aqo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.612 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 3.850 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.340 | |
| Number of reflections | 25900 | 4621 |
| <I/σ(I)> | 4.8 | 1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6 | 6.1 |
| CC(1/2) | 0.990 | 0.178 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 291 | 0.225 M MES/bis-tris pH 6.6 and 6.6 % w/v PEG 6000 |
