6F8T
Crystal structure of the PDE4D catalytic domain in complex with GEBR-4a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.531, 98.717, 119.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.627 - 1.800 |
R-factor | 0.1725 |
Rwork | 0.170 |
R-free | 0.21170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f6u |
RMSD bond length | 0.006 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.627 | 2.247 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | |
Number of reflections | 71439 | |
<I/σ(I)> | 10 | |
Completeness [%] | 99.9 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Hepes PEG 3350 Magnesium Chloride |