6F5H
Crystal structure of USP7 in complex with a 4-hydroxypiperidine based inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-01-27 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.865, 67.328, 80.686 |
| Unit cell angles | 90.00, 105.06, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.160 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.26240 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmeas | 0.110 | 0.670 |
| Number of reflections | 39952 | 5872 |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 95.9 | 96.8 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 4000, Tris, Li2-Sulfate, pH 7.75 |
