6F4T

Human JMJD5 (W414C) in complex with Mn(II), NOG and RCCD1 (139-143) (complex-5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.137, 65.169, 78.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.234 - 1.220
R-factor	0.1419
Rwork	0.141
R-free	0.15440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f4r
RMSD bond length	0.018
RMSD bond angle	1.511
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.130	1.290
High resolution limit [Å]	1.220	1.220
Rmerge	0.049	1.036
Rpim	0.023	0.481
Number of reflections	75316	10919
<I/σ(I)>	13.3	1.8
Completeness [%]	99.6	99.9
Redundancy	5.6
CC(1/2)	0.999	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M Bis-Tris pH 6.5, 17.1 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:1 ratio)