6F0W

prolyl hydroxylase in complex with hypoxia inducible factor oxygen degradation domain peptide fragment from Trichoplax adhaerens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	40.841, 41.321, 42.076
Unit cell angles	114.06, 95.67, 103.69

Refinement procedure

Resolution	37.478 - 1.301
R-factor	0.1389
Rwork	0.138
R-free	0.16220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hqr
RMSD bond length	0.012
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.112	0.065
Total number of observations	377792
Number of reflections	55428	5723	5382
<I/σ(I)>	8.5
Completeness [%]	95.1	98.4	92.3
Redundancy	6.8	6.8	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20 mg/ml protein, 1mM MN(II)CL2, 2mM N-oxalylglycine, 10mM taHIF-ODD peptide, 0.31M ammonium acetate, 24% (w/v) PEG3350, 0.1M Bis-Tris pH 5.5