6ERH
Complex of XLF and heterodimer Ku bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98015 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.847, 118.925, 128.219 |
| Unit cell angles | 90.00, 93.11, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jey |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.380 | 3.160 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.164 | 1.350 |
| Rmeas | 0.182 | 1.470 |
| Rpim | 0.094 | 0.760 |
| Number of reflections | 41644 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 50.4 | 8.5 |
| Redundancy | 7.1 | 6.9 |
| CC(1/2) | 0.997 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 18% PEG 3350, 150 mM sodium sulfate, and 100 mM Bis-Tris-Propane |
