6ERG
Complex of XLF and heterodimer Ku bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98009 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 111.710, 114.260, 127.170 |
Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.900 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jey |
RMSD bond length | 0.007 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.760 | 3.090 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.171 | 2.480 |
Rmeas | 0.182 | 2.560 |
Rpim | 0.068 | 0.940 |
Number of reflections | 51644 | |
<I/σ(I)> | 9.7 | 1 |
Completeness [%] | 72.9 | 21 |
Redundancy | 14 | 14.2 |
CC(1/2) | 0.996 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 18% PEG 3350, 150 mM Na sulfate , 100 mM Bis-Tris-Propane |