6EK6
Crystal structure of KDM5B in complex with S49195a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.410, 141.410, 150.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.742 - 2.050 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.867 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.030 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.125 | |
| Number of reflections | 56258 | 4073 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.6 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic |
