6EJ5

A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 3
Unit cell lengths	146.852, 146.852, 146.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.620 - 3.340
R-factor	0.2859
Rwork	0.284
R-free	0.30910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xzp
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.620	3.610
High resolution limit [Å]	3.340	3.340
Rmerge	0.099	0.800
Number of reflections	15480	1512
<I/σ(I)>	13.9	2.3
Completeness [%]	99.2	98.9
Redundancy	6.7	6.9
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	1.4 M sodium malonate, 50 mM sodium citrate pH 6.2