6EHO

Dimer of the Sortilin Vps10p domain at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-12-05
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 21 3
Unit cell lengths	189.010, 189.010, 189.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.505 - 3.500
R-factor	0.2333
Rwork	0.232
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f6k
RMSD bond length	0.002
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.505	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.052	0.590
Number of reflections	14135
<I/σ(I)>	17.4
Completeness [%]	98.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	2.0 Ammonium sulphate