6EAQ

Glycosylated FCGR3B / CD16b in complex with afucosylated IgG1 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	123.629, 49.388, 139.415
Unit cell angles	90.00, 103.04, 90.00

Refinement procedure

Resolution	45.695 - 2.220
R-factor	0.2153
Rwork	0.213
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vu0
RMSD bond length	0.010
RMSD bond angle	1.073
Data reduction software	HKL-3000 (Rv704w)
Data scaling software	HKL-3000 (Rv704w)
Phasing software	PHASER (1.10.1-2155)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.695	2.299
High resolution limit [Å]	2.220	2.220
Rmerge	0.177	2.410
Number of reflections	40439	3536
<I/σ(I)>	7.01
Completeness [%]	97.0
Redundancy	3.5
CC(1/2)	0.974	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	pH 6.0 50 mM MES, 8% PEG3350 and 60 mM NaCl