6EA5

Structure of BDBV GPcl in complex with the pan-ebolavirus mAb ADI-15878

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03321
Spacegroup name	P 31 2 1
Unit cell lengths	151.625, 151.625, 247.098
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.260 - 4.750
R-factor	0.296
Rwork	0.295
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hj3
RMSD bond length	0.006
RMSD bond angle	1.142
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (DEV_1539)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.770	5.310
High resolution limit [Å]	4.750	4.750
Rmerge	0.090
Number of reflections	17080	4757
<I/σ(I)>	10.1	1.1
Completeness [%]	99.7	100
Redundancy	6.5	6.5
CC(1/2)		0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	2% Tacsimate pH 8.0, 100 mM Tris pH 8.5, and 16% PEG 3350