6E67
Structure of beta2 adrenergic receptor fused to a Gs peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-05-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.010, 377.440, 45.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 3.700 |
R-factor | 0.2794 |
Rwork | 0.276 |
R-free | 0.30720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lde |
RMSD bond length | 0.003 |
RMSD bond angle | 0.433 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Number of reflections | 16163 | 1257 |
<I/σ(I)> | 4.15 | 1.05 |
Completeness [%] | 99.0 | 98.8 |
Redundancy | 7.5 | 4.5 |
CC(1/2) | 0.980 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol |