6E67

Structure of beta2 adrenergic receptor fused to a Gs peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-2 adrenergic receptor,Endolysin,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Beta-2 adrenergic receptor chimera	polymer	531	60158.5	2	UniProt (P07550) UniProt (A0A097J809) UniProt (P63092) Pfam (PF00001) Pfam (PF00959)	Homo sapiens (Human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor,Lysis protein,Lysozyme,Muramidase,Adenylate cyclase-stimulating G alpha protein,Beta-2 adrenoreceptor,Beta-2 adrenoceptor
2	C, D (A, B)	8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one	non-polymer		370.4	2	Chemie (P0G)

Sequence modifications

A, B: 1 - 232 (UniProt: P07550)

PDB	External Database	Details
Met -23	-	expression tag
Lys -22	-	expression tag
Thr -21	-	expression tag
Ile -20	-	expression tag
Ile -19	-	expression tag
Ala -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Tyr -15	-	expression tag
Ile -14	-	expression tag
Phe -13	-	expression tag
Cys -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Phe -9	-	expression tag
Ala -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Arg 16	Gly 16	variant
Gln 27	Glu 27	variant
Thr 96	Met 96	engineered mutation
Thr 98	Met 98	engineered mutation
Glu 187	Asn 187	engineered mutation
Cys 226	Ala 226	engineered mutation

A, B: 1002 - 1161 (UniProt: A0A097J809)

PDB	External Database	Details
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation

A, B: 2381 - 2394 (UniProt: P63092)

PDB	External Database	Details
Cys 2394	Leu 394	linker
Gly 3001	-	linker
Thr 3002	-	linker
Gly 3003	-	linker

A, B: 268 - 365 (UniProt: P07550)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120317.1
Non-Polymers*	Number of molecules	2
	Total formula weight	740.9
All*	Total formula weight	121058.0

*Water molecules are not included.