6E3U
Crystal Structure of the Heterodimeric HIF-2 Complex with Agonist M1001
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.951, 98.846, 77.077 |
Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
Resolution | 46.126 - 2.850 |
R-factor | 0.2015 |
Rwork | 0.199 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zp4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.093 | 0.741 |
Number of reflections | 16131 | 818 |
<I/σ(I)> | 15.3 | 1.7 |
Completeness [%] | 98.0 | 97.7 |
Redundancy | 4.6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 2% TACSIMATE, PH 7.0, 6% PEG3350 |