6E3S
Crystal Structure of the Heterodimeric HIF-2 Complex with Antagonist PT2385
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.009, 97.895, 77.965 |
Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
Resolution | 48.948 - 3.000 |
R-factor | 0.2139 |
Rwork | 0.212 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zp4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.257 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.099 | 0.765 |
Number of reflections | 14037 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.0 | |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2% TACSIMATE, PH 7.0, 6% PEG3350 |