6E2Q

Structure of human JAK2 FERM/SH2 in complex with Erythropoietin Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	C 1 2 1
Unit cell lengths	178.490, 114.880, 179.820
Unit cell angles	90.00, 93.21, 90.00

Refinement procedure

Resolution	48.440 - 2.650
R-factor	0.226
Rwork	0.225
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z32
RMSD bond length	0.001
RMSD bond angle	0.377
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.745
High resolution limit [Å]	2.650	2.650
Rmerge	0.073	0.865
Rmeas	0.087	1.031
Rpim	0.046	0.555
Number of reflections	104923	10236
<I/σ(I)>	13.1	1.25
Completeness [%]	99.5	97.9
Redundancy	3.4	3.3
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277.15	100 mM Tris, pH 7.6, 2-4% PEG8000