6DNA
Crystal structure of T110A mutant human Glutamate oxaloacetate transaminase 1 (GOT1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 257.700, 148.626, 83.183 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 48.700 - 3.000 |
| R-factor | 0.18538 |
| Rwork | 0.184 |
| R-free | 0.21144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ii0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.465 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.610 |
| Number of reflections | 4281 | |
| <I/σ(I)> | 9.42 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 25% w/v PEG 3,350, 0.1 M HEPES pH 7.5 |
