6DG5

Structure of a de novo designed Interleukin-2/Interleukin-15 mimetic complex with IL-2Rb and IL-2Rg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 2 21
Unit cell lengths	65.125, 67.914, 172.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.005 - 2.516
R-factor	0.2256
Rwork	0.221
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5i
Data reduction software	XDS (20171111)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.005	2.828
High resolution limit [Å]	2.516	2.516
Rmerge	0.461	2.516
Rmeas	0.488	5.756
Rpim	0.158	0.780
Total number of observations	163261
Number of reflections	17512	698
<I/σ(I)>	5.6
Completeness [%]	100.0	9
Redundancy	9.3	11.22
CC(1/2)	0.983	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.091 M Tris pH 7.5 8 mM imidazole pH 8.0 4.2% DEXTRAN SULFATE 50 mM sodium citrate 2.2 M ammonium sulfate