6DCA
Fab/epitope complex of mouse monoclonal antibody 6B2 targeting a non-phosphorylated tau epitope.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-BM-B |
Synchrotron site | APS |
Beamline | 23-BM-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 54.010, 59.704, 143.846 |
Unit cell angles | 89.43, 92.69, 97.35 |
Refinement procedure
Resolution | 45.605 - 2.599 |
R-factor | 0.1914 |
Rwork | 0.189 |
R-free | 0.25880 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.605 | 2.690 |
High resolution limit [Å] | 2.602 | 2.602 |
Number of reflections | 53304 | |
<I/σ(I)> | 5.84 | |
Completeness [%] | 97.6 | |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2M ammonium sulfate |