6DCA

Fab/epitope complex of mouse monoclonal antibody 6B2 targeting a non-phosphorylated tau epitope.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	54.010, 59.704, 143.846
Unit cell angles	89.43, 92.69, 97.35

Refinement procedure

Resolution	45.605 - 2.599
R-factor	0.1914
Rwork	0.189
R-free	0.25880
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.605	2.690
High resolution limit [Å]	2.602	2.602
Number of reflections	53304
<I/σ(I)>	5.84
Completeness [%]	97.6
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2M ammonium sulfate