6DC8

Fab/epitope complex of mouse monoclonal antibody 8B2 targeting a non-phosphorylated tau epitope.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	68.836, 68.836, 198.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.302 - 1.799
R-factor	0.1811
Rwork	0.179
R-free	0.21870
RMSD bond length	0.006
RMSD bond angle	0.912
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	1.860
High resolution limit [Å]	1.790	1.790
Number of reflections	45390
<I/σ(I)>	14.5
Completeness [%]	99.9
Redundancy	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	23% polyethylene glycol 8000, 0.17 M ammonium acetate, and 0.085 M sodium cacodylate trihydrate pH 6.5