6D2P
Crystal structure of IOMA-class CLK31 Fab from an HIV-1 naive donor in complex with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.6 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-10-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.300, 66.636, 76.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.259 - 2.600 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.26760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ies |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Data reduction software | HKL-2000 (705b) |
Data scaling software | HKL-2000 (705b) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.260 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 21985 | 2165 |
<I/σ(I)> | 7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.9 | 10.8 |
CC(1/2) | 0.920 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | pH 5.0 of 20% w/v PEG6000, 0.1M citric acid (pH 4.0) |