6D06

Human ADAR2d E488Y mutant complexed with dsRNA containing an abasic site opposite the edited base

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	174.680, 63.440, 132.060
Unit cell angles	90.00, 126.64, 90.00

Refinement procedure

Resolution	105.960 - 2.550
R-factor	0.19334
Rwork	0.190
R-free	0.25518
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zy7
RMSD bond length	0.012
RMSD bond angle	1.739
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.000	2.620
High resolution limit [Å]	2.550	2.550
Rmerge	0.063	0.723
Number of reflections	37329	2749
<I/σ(I)>	15.27	2.07
Completeness [%]	97.4	98.1
Redundancy	3.44	3.5
CC(1/2)	0.998	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M MES:NaOH pH 6.5, 14% PEG 20,000