6CBV
Crystal structure of BRIL bound to an affinity matured synthetic antibody.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979100 |
Spacegroup name | P 65 |
Unit cell lengths | 87.954, 87.954, 159.107 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 76.171 | 76.171 | 1.990 |
High resolution limit [Å] | 1.870 | 5.600 | 1.870 |
Rmerge | 0.041 | 0.023 | 0.892 |
Rmeas | 0.045 | 0.026 | 1.106 |
Total number of observations | 263005 | ||
Number of reflections | 55287 | 2165 | 7533 |
<I/σ(I)> | 17.55 | 58.12 | 0.94 |
Completeness [%] | 96.7 | 99.4 | 81.6 |
Redundancy | 4.757 | 5.155 | 2.538 |
CC(1/2) | 0.999 | 0.999 | 0.461 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Sodium formate, 20 % w/v PEG 3350 |