6C4J

Ligand bound full length hUGDH with A104L substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-25
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	403.550, 112.590, 184.670
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	48.632 - 2.530
R-factor	0.1819
Rwork	0.180
R-free	0.21410
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.559
High resolution limit [Å]	2.530	2.530
Number of reflections	272746
<I/σ(I)>	9.81
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3 mM UDP-Glc, 5mM NADH, PEG 3350, Ammonium Sulfate, NaCl, 1,2 propanediol, Tris pH (8.6)