6C2Y

Human GRK2 in complex with Gbetagamma subunits and CCG257142

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	60.557, 240.453, 212.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.320 - 2.740
R-factor	0.2466
Rwork	0.244
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pnk
RMSD bond length	0.001
RMSD bond angle	0.439
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.320	2.838
High resolution limit [Å]	2.740	2.740
Rmerge	0.304	2.061
Rmeas	0.316	2.137
Number of reflections	40501	4026
<I/σ(I)>	5.62	1.17
Completeness [%]	96.0	99
Redundancy	14.1	14.6
CC(1/2)	0.991	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES pH 6.0, 800 mM NaCl, 8% PEG 3350