6C19
FGFR1 kinase complex with inhibitor SN36985
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-01-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.426, 49.013, 64.628 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 19.710 - 2.120 |
| R-factor | 0.20704 |
| Rwork | 0.205 |
| R-free | 0.23852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wun |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.710 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.080 | 0.682 |
| Rpim | 0.034 | 0.293 |
| Number of reflections | 21315 | 2944 |
| <I/σ(I)> | 19.9 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% MPEG 5000, 0.1 M sodium cacodylate pH 7.5, 0.2 M ammonium sulfate |
