6BVB

Crystal structure of HIF-2alpha-pVHL-elongin B-elongin C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28339
Spacegroup name	P 43 21 2
Unit cell lengths	59.770, 59.770, 246.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.307 - 2.002
R-factor	0.2005
Rwork	0.199
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lm8
RMSD bond length	0.012
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.307	2.050
High resolution limit [Å]	2.002	2.002
Rmeas	0.095	0.912
Number of reflections	31053	2084
<I/σ(I)>	13.2	1.32
Completeness [%]	99.3	92.2
Redundancy	8.54	3.81
CC(1/2)	0.997	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	26% (v/v) PEG-3350, 0.2 M Li2SO4, 0.1 M Bis-Tris. 7 mg/mL protein complex.