6BS0
JAK2 JH2 in complex with 63552444
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.407, 57.494, 61.082 |
Unit cell angles | 90.00, 110.47, 90.00 |
Refinement procedure
Resolution | 41.602 - 1.541 |
R-factor | 0.1618 |
Rwork | 0.160 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fvp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.244 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.540 |
Number of reflections | 42449 | |
<I/σ(I)> | 26.4 | |
Completeness [%] | 99.8 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris, pH 8.0 0.2M Sodium acetate 12-20% PEG 4,000 |