6BRW
JAK2 JH2 in complex with XMU-MP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.386, 57.147, 60.398 |
| Unit cell angles | 90.00, 110.24, 90.00 |
Refinement procedure
| Resolution | 41.646 - 2.031 |
| R-factor | 0.1968 |
| Rwork | 0.194 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.226 | 0.175 |
| Number of reflections | 18266 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris, pH 8.0 0.2M Sodium acetate 12-20% PEG 4,000 |
