6B67
Human PP2Calpha (PPM1A) complexed with cyclic peptide c(MpSIpYVA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-07-06 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.030, 89.570, 70.050 |
Unit cell angles | 90.00, 113.86, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6q |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 43867 | |
<I/σ(I)> | 25.09 | 4.63 |
Completeness [%] | 95.7 | |
Redundancy | 2.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | Protein conc: 20 mg/ml Protein buffer: 10 mM MES, pH 7.0, 150 mM NaCl, 2 mM TCEP and 2 mM MgCl2 Protein and cyclic peptide was dialyzed against above buffer and mixed at the 1:2 molar ratio Precipitant: 0.1M HEPES (pH 7.3), 0.1M calcium acetate and 40% PEG400 |