6AMB
Crystal Structure of the Afadin RA1 domain in complex with HRAS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.239, 57.152, 73.329 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.301 - 2.500 |
R-factor | 0.2232 |
Rwork | 0.218 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ddc |
RMSD bond length | 0.002 |
RMSD bond angle | 0.522 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.097 | 0.069 | 0.557 |
Rmeas | 0.106 | 0.076 | 0.609 |
Rpim | 0.041 | 0.031 | 0.241 |
Number of reflections | 7437 | 427 | 358 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 98.8 | 100 |
Redundancy | 6.9 | 5.9 | 6.2 |
CC(1/2) | 0.996 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.9 | 297 | PEG3350, K2HPO4 |