6AKO
Crystal Structure of FOXC2 DBD Bound to DBE2 DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2016-03-06 |
Detector | RIGAKU |
Wavelength(s) | 1.5870 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.540, 72.924, 99.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.970 - 2.396 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x07 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.611 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.970 | 2.482 |
High resolution limit [Å] | 2.396 | 2.396 |
Rmerge | 0.041 | 0.302 |
Number of reflections | 11726 | 780 |
<I/σ(I)> | 34.65 | |
Completeness [%] | 92.0 | |
Redundancy | 12.1 | |
CC(1/2) | 1.000 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Bis-Tris propane (pH 6.68), 14% PEG4K, 200 mM NaCl, 10 mM MgCl2, 1 mM TCEP |