6A4B
Structure of TREX2 in complex with a duplex DNA with 2 nucleotide 3'-overhang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.715, 87.317, 432.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.233 - 2.700 |
| R-factor | 0.2274 |
| Rwork | 0.224 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y97 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.067 | 0.486 |
| Rpim | 0.040 | 0.310 |
| Number of reflections | 51078 | 4998 |
| <I/σ(I)> | 23.45 | 1.93 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Sodium citrate tribasic dihydrate pH 5.5, 22%(w/v) Polyethylene glycol 1,000, 0.1M Barium chloride dihydrate |
