6A46
Structure of TREX2 in complex with a nucleotide (dCMP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.808, 54.773, 88.716 |
| Unit cell angles | 90.00, 100.76, 90.00 |
Refinement procedure
| Resolution | 28.859 - 2.000 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y97 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.474 |
| Rpim | 0.033 | 0.344 |
| Number of reflections | 27839 | 2726 |
| <I/σ(I)> | 22.37 | 1.875 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.02M Nickel(II) chloride hexahydrate, 0.02M Magnesium chloride hexahydrate, 0.02M Cadmium chloride hydrate, 0.1M Sodium acetate trihydrate pH 4.5, 24%(w/v) Polyethylene glycol monomethyl ether 2,000 |
